Wildlife Crossings

ALTO HSR · Citizen Research · Environmental Analysis

The Wildlife Crossing Problem

What do wildlife crossings and underpasses actually cost, what does the international HSR evidence say about whether they work — and are the A2A Collaborative’s mitigation recommendations adequate, or even likely to be adopted?

⚠ What this research shows

A new high-speed rail line is an absolute linear barrier — fully fenced, grade-separated, and impassable. Mitigation through wildlife crossings is possible but expensive, imperfect, and species-specific. On the Frontenac Arch section of ALTO’s southern corridor, the scale of adequate mitigation is far larger than anything ALTO’s consultation documents acknowledge. The most ambitious independent programme proposed — that of the A2A Collaborative — would, by A2A’s own admission, fail to fully restore connectivity.

Key Finding

The A2A Collaborative’s mitigation recommendations would cost hundreds of millions of dollars for the Frontenac Arch section alone, are unlikely to be adopted at that density under a DBFOM P3 structure, and would still fail to fully restore ecological connectivity for many species.

For several SARA-listed species — the Eastern Loggerhead Shrike, four bat species at the Moira Karst hibernaculum, and the karst-dependent Grey Ratsnake — crossing structures cannot mitigate the harm at all. Route avoidance is the only adequate response for those species.

Benchmark Evidence

What a wildlife crossing actually costs

Wildlife crossing structures range enormously in scale and cost — from small amphibian culverts costing tens of thousands of dollars to major vegetated overpasses exceeding $100 million. Four benchmark cases illustrate the cost range for structures capable of accommodating large mammals.

Wallis Annenberg Wildlife Crossing — California (US 101)

Originally budgeted at $92M for a single vegetated overpass spanning ten lanes of the 101 Freeway. Ran $21M over budget by early 2026 — a 23% overrun — bringing the total to approximately $114M. Expected completion late 2026, four years after groundbreaking. The 2018 estimate for the same project was $4M — a 2,750% underestimate at 30% design stage.

Caltrans / Governor of California, 2022–2026

I-25 Greenland Wildlife Overpass — Colorado (6-lane interstate)

At 200 × 209 feet, North America’s largest wildlife overpass by surface area. Completed December 2025 on time and under budget at $15M. Specifically designed for elk and pronghorn — large mammals that refuse enclosed underpasses. Expected to reduce wildlife-vehicle collisions by 90%. Part of a 30-year, $300M+ corridor investment.

Colorado Department of Transportation, 2025

Ojibway Parkway Wildlife Crossing — Windsor, Ontario

Preferred option estimated at $13M. Critical limitation noted in planning documents: the crossing stops at the railway tracks that border the parkway, providing no crossing of the railway itself. The railway is identified as a separate and unresolved barrier. This is the exact planning gap ALTO reproduces — road crossings are costed; railway barriers are deferred.

Windsor Star, 2025

Banff National Park System — Trans-Canada Highway

44 crossing structures + 88 km of exclusion fencing along 82 km of highway. Built over 25 years at a total corridor investment exceeding $300M. Wildlife-vehicle collisions dropped 80% overall, 96% for elk and deer. This system is on a 4-lane highway operating at 110 km/h — not a 300 km/h fenced HSR line with up to 72 trains per day.

Clevenger & Waltho, 2005; TransCanada data
Structure typeTypical cost (USD)Target speciesHSR suitability
Small amphibian/reptile culvert$50K – $200KFrogs, snakes, turtlesPartial — needed every 300–500m; does not address large mammals
Wildlife underpass (medium)$500K – $2.7MDeer, bears, medium mammalsLimited — elk avoid enclosed tunnels; effective for mesopredators if correctly sized
Large vegetated landscape bridge$15M – $30MElk, bear, wolf, full communityMost effective; European standard for HSR through sensitive corridors
Major ecoduct (Netherlands standard)$50M – $114M+Full species communityGold standard; Zanderij Crailoo (800m, spans highway + railway) is the benchmark
Elevated viaduct (per km, HSR)$30M – $80M/kmAll species — natural movement underneathEliminates barrier for most species; A2A recommends for most sensitive areas
Exclusion fencing (per km, both sides)$100K – $350K/kmAll species — mandatory for HSRWithout crossings, creates absolute permanent barrier; funnels animals toward adjacent roads

Costs in USD; Canadian equivalent approximately 35–40% higher. Costs from Large Landscapes Coalition (2021), CDOT (2025), Caltrans (2026).

International Evidence

What other countries have learned from HSR and wildlife

92

Vertebrate deaths per km/yr on HSR

vs. 36.5 on conventional rail — a 2.5× increase (Spanish HSR study, cited by A2A 2026)

646

Wildlife deaths at Banff (24 years)

On a conventional freight railway — including 106 bears. ALTO proposes 300 km/h and up to 72 trains/day

0

Deer/wild boar crossings recorded

In 2-year study of 15 underpasses + 2 overpasses on a 25 km Spanish HSR section (Rodriguez et al. 1996)

10+ km

Sensory impact radius

A2A primer: impacts “extend up to over 10km.” Crossings address physical barrier; not noise and vibration

600+

Wildlife tunnels in Netherlands

Under roads and railways since 1988. Includes the 800m Zanderij Crailoo ecoduct spanning highway + railway

25 yrs

Time to build Banff system

Infrastructure of this complexity requires commitment before construction — not as an afterthought

A2A Collaborative’s own admission — February 2026

The A2A Collaborative’s ecological primer states directly: “Without (and potentially even with) robust mitigation, rail will significantly impact connectivity, necessitating new connectivity studies after the rail is completed and potential remapping of protected areas.” That is the organisation advocating for the Frontenac Arch corridor acknowledging that even its own recommended mitigation programme may not be sufficient.

France: fencing and crossings as a planning precondition

France’s lignes à grande vitesse are fully fenced and require a “double series of measures” — full-length species-appropriate fencing plus ecological permeability structures at ecologically determined intervals. The UIC (International Union of Railways) 2011 report calls this “state of the art to fulfil all obligations in order to secure planning permission.” France treats this as a precondition, not a post-route mitigation option.

Spain: deer and wild boar crossed zero times

A study along 25 km of Spanish HSR monitored 15 underpasses and two overpasses over two years. Reptiles used the structures; deer and wild boar made zero crossings. Large ungulates consistently avoid enclosed tunnel structures. Only wide, open, vegetated overpasses at $15M–$30M+ each attract these species — and those are exactly the structures a DBFOM contractor has maximum incentive to eliminate.

The Frontenac Arch

Why ALTO’s corridor is especially difficult to mitigate

ALTO’s southern corridor traverses the Frontenac Arch — a 2,700 km² UNESCO Biosphere Reserve identified by conservation scientists as the most important remaining wildlife movement corridor in northeastern North America. The A2A Collaborative’s own primer describes it as a “Double Barrier” problem: an HSR line running just north of Highway 401 creates a trapped zone where animals surviving a highway crossing face immediate re-entrapment against HSR security fencing.

Nine Carleton University ecologists and conservation scientists, in an open letter released February 24, 2026, explicitly warned that the project “could disrupt ecological and hydrological connectivity in the Frontenac Arch Biosphere Reserve” and called for mitigation planning to begin before routes are finalized.

Species for which crossing structures are not sufficient

Eastern Loggerhead Shrike

SARA Endangered · ~40 wild individuals · ~22 breeding pairs at Napanee

Breeds within Napanee limestone plain habitat — it does not cross the rail line. Noise, vibration, and artificial light from HSR degrade habitat quality across a wide zone. No crossing structure is relevant to its survival.

Four SARA-Listed Bat Species

SARA Endangered / Threatened · Moira Karst hibernaculum

Bat foraging habitat within range of hibernacula is degraded by railway noise and vibration at HSR speeds. Wildlife crossings are entirely irrelevant — the problem is habitat quality, not physical crossing.

Grey Ratsnake

SARA Threatened · SARA Critical Habitat formally mapped

Critical habitat is mapped along the Frontenac Arch. Population connectivity requires multiple underpasses within each home range. A2A recommends reptile tunnels every 300–500m — for 100km of corridor, 200–333 structures.

Salmon River Alvar

Unique · Sole Canadian Juniper Sedge population

Alvar cannot be recreated or transplanted. Construction through or adjacent to this site constitutes permanent loss. Route avoidance is the only option — no crossing structure restores alvar habitat.

Blanding’s Turtle & Freshwater Turtles

SARA Threatened

Require seasonal overland movement between wetlands. Culverts can work if paired with drift fencing and placed based on pre-construction movement surveys — surveys ALTO has not conducted for this section.

Black Bear, Moose, White-tailed Deer

Ecologically critical for corridor function

The Frontenac Arch’s continental function depends on their movement. The Spanish HSR data shows these species require wide vegetated overpasses. Based on Banff data, adequate crossing density is approximately one structure per 2 km.

The A2A Programme

What A2A recommends — and what it would cost

The A2A Collaborative published a background ecological primer in February 2026 with specific mitigation standards. Their recommended crossing densities for the Frontenac Arch section:

Herpetile tunnels — every 300–500m

Dedicated amphibian/reptile tunnels throughout critical corridor sections to prevent entrapment and facilitate seasonal migrations. For 100km of sensitive corridor: 200–333 structures at $135K–$270K CAD each. Subtotal: $27M–$90M per 100km.

Medium mammal culverts — every 1–2km

Large box culverts for mesopredators and forest-dwelling species. For 100km: 50–100 structures at $675K–$3.6M CAD each. Subtotal: $34M–$360M per 100km.

Large mammal landscape bridges — every 5–8km

Massive vegetated overpasses for apex predators and large ungulates. For 100km: 12–20 structures at $20M–$40M CAD each. Subtotal: $240M–$800M per 100km.

Ecological buffer zones + viaducts

400m exclusion zones around forest interior and wetlands. Elevated viaduct construction through highest-sensitivity areas at $40M–$108M/km. For 10km of viaduct: $400M–$1,080M.

Total A2A-standard mitigation for a representative 100km of Frontenac Arch corridor: $728M – $2.4 billion — representing 1–4% of ALTO’s central cost estimate for one section alone.
⚠ The P3 structural incentive problem

ALTO is a DBFOM P3. Cadence — the consortium bearing construction cost risk — has a direct financial incentive to minimise crossing structures. Under a DBFOM structure, crossing commitments must be contractually specified before financial close. ALTO has made no such commitment. The public consultation closes April 24, 2026. No contract terms have been disclosed. No crossing density has been committed. ALTO’s stated position: mitigation will be determined “community-by-community after route selection.” That sequencing is the inverse of what international standards, the A2A, and nine Carleton University ecologists all require.

Our Assessment

Will ALTO mitigate sufficiently? Almost certainly not.

The financial and structural forces against full adoption of the A2A programme are severe. A partial programme — some crossings, well below A2A’s standard — is the most likely outcome. That is not adequate for the Frontenac Arch.
Even if ALTO adopted every A2A recommendation, the outcome would be significant net harm. The double-barrier problem, the sensory barrier extending 10+ km on each side, and species-specific impacts on shrikes, bats, and alvar cannot be mitigated by crossing structures. A2A’s own primer acknowledges this.
The Spanish HSR data in A2A’s primer shows 92 vertebrate deaths per km/yr on HSR. For 100km of Frontenac Arch corridor, that projects to approximately 9,200 vertebrate deaths annually — every year, indefinitely.
For the Eastern Loggerhead Shrike, four SARA-listed bat species, the Salmon River Alvar, and Stone Mills karst aquifers — route avoidance is the only adequate response. Crossing structures cannot address these impacts.
Adequate mitigation requires commitments before route selection, not after. ALTO has not made those commitments. The consultation closes April 24, 2026.
Submit your comments by April 24, 2026 →
Key Sources

Research underpinning this brief

1
A2A Collaborative. Background Primer on Ecological Impacts of the ALTO High Speed Rail in the A2A Corridor. February 2026.
2
Friends of Napanee River. Submission to the ALTO High Speed Rail Project Opposing Both Options. March 2026.
3
St. Clair, C.C. et al. “Railway mortality for several mammal species increases with train speed, proximity to water, and track curvature.” Scientific Reports, 10(1), 2020.
4
Rodriguez, A. et al. Study of wildlife crossings on 25 km Spanish HSR section, Castilla-La Mancha. Biological Conservation, 1996.
5
UIC (International Union of Railways). High Speed Rail and Sustainability. 2011.
6
Colorado Department of Transportation. I-25 Greenland Wildlife Overpass completion report, December 2025. Final cost $15M.
7
California Governor’s Office / Caltrans. Wallis Annenberg Wildlife Crossing records, 2022–2026. Original $92M budget; current $114M. 101wildlifecrossing.org
8
Carleton University Ecologists (9 co-signatories). Open letter on ALTO HSR environmental impacts, February 24, 2026.
9
A2A Collaborative / Frontenac Arch Safe Passage. Right to Roam report, 2025–2026. frontenacsafepassage.org
10
Alto HSR Corporate Communications. Crystal Jogenward statement, March 9, 2026: “We must first make the route selection… mitigation strategies… determined on a community-by-community basis.”